diff --git a/.changeset/hot-readers-promise.md b/.changeset/hot-readers-promise.md
new file mode 100644
index 00000000000..8416b7f284b
--- /dev/null
+++ b/.changeset/hot-readers-promise.md
@@ -0,0 +1,7 @@
+---
+'@primer/react': minor
+---
+
+Add option to specify custom widths for PageLayout.Pane via the `width` prop.
+
+<!-- Changed components: PageLayout -->
diff --git a/src/PageLayout/PageLayout.docs.json b/src/PageLayout/PageLayout.docs.json
index dfa273638c5..14cf798687e 100644
--- a/src/PageLayout/PageLayout.docs.json
+++ b/src/PageLayout/PageLayout.docs.json
@@ -148,9 +148,9 @@
         },
         {
           "name": "width",
-          "type": "| 'small' | 'medium' | 'large'",
+          "type": "| 'small' | 'medium' | 'large' | { min: string max: string default: string }",
           "defaultValue": "'medium'",
-          "description": "The width of the pane."
+          "description": "The width of the pane. If using custom widths, provide an object with keys 'min', 'max' and 'default'."
         },
         {
           "name": "minWidth",
diff --git a/src/PageLayout/PageLayout.features.stories.tsx b/src/PageLayout/PageLayout.features.stories.tsx
index 9a168985c56..9f2ceebed5f 100644
--- a/src/PageLayout/PageLayout.features.stories.tsx
+++ b/src/PageLayout/PageLayout.features.stories.tsx
@@ -340,3 +340,20 @@ export const ScrollContainerWithinPageLayoutPane: Story = () => (
     </Box>
   </Box>
 )
+
+export const CustomPaneWidths: Story = () => (
+  <PageLayout containerWidth="full">
+    <PageLayout.Header>
+      <Placeholder height={64} label="Header" />
+    </PageLayout.Header>
+    <PageLayout.Pane resizable width={{min: '200px', default: '300px', max: '400px'}}>
+      <Placeholder height={320} label="Pane" />
+    </PageLayout.Pane>
+    <PageLayout.Content>
+      <Placeholder height={640} label="Content" />
+    </PageLayout.Content>
+    <PageLayout.Footer>
+      <Placeholder height={64} label="Footer" />
+    </PageLayout.Footer>
+  </PageLayout>
+)
diff --git a/src/PageLayout/PageLayout.tsx b/src/PageLayout/PageLayout.tsx
index 48533afc22e..71371fc4233 100644
--- a/src/PageLayout/PageLayout.tsx
+++ b/src/PageLayout/PageLayout.tsx
@@ -466,6 +466,25 @@ Content.displayName = 'PageLayout.Content'
 // ----------------------------------------------------------------------------
 // PageLayout.Pane
 
+type Measurement = `${number}px`
+
+type CustomWidthOptions = {
+  min: Measurement
+  default: Measurement
+  max: Measurement
+}
+
+type PaneWidth = keyof typeof paneWidths
+
+const isCustomWidthOptions = (width: PaneWidth | CustomWidthOptions): width is CustomWidthOptions => {
+  // eslint-disable-next-line @typescript-eslint/no-unnecessary-condition
+  return (width as CustomWidthOptions).default !== undefined
+}
+
+const isPaneWidth = (width: PaneWidth | CustomWidthOptions): width is PaneWidth => {
+  return ['small', 'medium', 'large'].includes(width as PaneWidth)
+}
+
 export type PageLayoutPaneProps = {
   position?: keyof typeof panePositions | ResponsiveValue<keyof typeof panePositions>
   /**
@@ -485,7 +504,7 @@ export type PageLayoutPaneProps = {
   positionWhenNarrow?: 'inherit' | keyof typeof panePositions
   'aria-labelledby'?: string
   'aria-label'?: string
-  width?: keyof typeof paneWidths
+  width?: PaneWidth | CustomWidthOptions
   minWidth?: number
   resizable?: boolean
   widthStorageKey?: string
@@ -576,9 +595,18 @@ const Pane = React.forwardRef<HTMLDivElement, React.PropsWithChildren<PageLayout
       }
     }, [sticky, enableStickyPane, disableStickyPane, offsetHeader])
 
+    const getDefaultPaneWidth = (width: PaneWidth | CustomWidthOptions): number => {
+      if (isPaneWidth(width)) {
+        return defaultPaneWidth[width]
+      } else if (isCustomWidthOptions(width)) {
+        return Number(width.default.split('px')[0])
+      }
+      return 0
+    }
+
     const [paneWidth, setPaneWidth] = React.useState(() => {
       if (!canUseDOM) {
-        return defaultPaneWidth[width]
+        return getDefaultPaneWidth(width)
       }
 
       let storedWidth
@@ -589,7 +617,7 @@ const Pane = React.forwardRef<HTMLDivElement, React.PropsWithChildren<PageLayout
         storedWidth = null
       }
 
-      return storedWidth && !isNaN(Number(storedWidth)) ? Number(storedWidth) : defaultPaneWidth[width]
+      return storedWidth && !isNaN(Number(storedWidth)) ? Number(storedWidth) : getDefaultPaneWidth(width)
     })
 
     const updatePaneWidth = (width: number) => {
@@ -733,7 +761,7 @@ const Pane = React.forwardRef<HTMLDivElement, React.PropsWithChildren<PageLayout
             updatePaneWidth(paneRect.width)
           }}
           // Reset pane width on double click
-          onDoubleClick={() => updatePaneWidth(defaultPaneWidth[width])}
+          onDoubleClick={() => updatePaneWidth(getDefaultPaneWidth(width))}
         />
 
         <Box
@@ -743,12 +771,14 @@ const Pane = React.forwardRef<HTMLDivElement, React.PropsWithChildren<PageLayout
             '--pane-width': `${paneWidth}px`,
           }}
           sx={(theme: Theme) => ({
-            '--pane-min-width': `${minWidth}px`,
+            '--pane-min-width': isCustomWidthOptions(width) ? width.min : `${minWidth}px`,
             '--pane-max-width-diff': '511px',
-            '--pane-max-width': `calc(100vw - var(--pane-max-width-diff))`,
+            '--pane-max-width': isCustomWidthOptions(width) ? width.max : `calc(100vw - var(--pane-max-width-diff))`,
             width: resizable
               ? ['100%', null, 'clamp(var(--pane-min-width), var(--pane-width), var(--pane-max-width))']
-              : paneWidths[width],
+              : isPaneWidth(width)
+              ? paneWidths[width]
+              : width.default,
             padding: SPACING_MAP[padding],
             overflow: [null, null, 'auto'],