diff --git a/CHANGELOG.md b/CHANGELOG.md
index 04c98ae6..3a821c14 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -4,6 +4,15 @@ All notable changes to this project will be documented in this file.
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/),
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## [Unreleased]
+
+### Removed
+
+- `rsconnect-python` no longer considers the `RETICULATE_PYTHON` environment variable.
+  In environments where `RETICULATE_PYTHON` is set outside a project context (e.g. by a Posit Workbench administrator),
+  attempting to deploy content or write manifests in projects using virtual environments required explicitly setting `--python /path/to/virtualenv/python`.
+  Removing `RETICULATE_PYTHON` detection should simplify the use of the CLI in this case.
+
 ## [1.13.0] - 2022-12-02
 
 ### Added
diff --git a/README.md b/README.md
index 46e61f9e..32f98f22 100644
--- a/README.md
+++ b/README.md
@@ -220,8 +220,7 @@ specified package list.
 
 If there is no `requirements.txt` file or the `--force-generate` option is specified,
 the package dependencies will be determined from the current Python environment, or
-from an alternative Python executable specified via the `--python` option or via the
-`RETICULATE_PYTHON` environment variable:
+from an alternative Python executable specified via the `--python` option:
 
 ```bash
 rsconnect deploy notebook --python /path/to/python my-notebook.ipynb
@@ -255,8 +254,7 @@ in a later deployment. Use the `write-manifest` command to do this.
 The `write-manifest` command will also create a `requirements.txt` file, if it does
 not already exist or the `--force-generate` option is specified. It will contain the
 package dependencies from the current Python environment, or from an alternative
-Python executable specified in the `--python` option or via the `RETICULATE_PYTHON`
-environment variable.
+Python executable specified in the `--python` option.
 
 Here is an example of the `write-manifest` command:
 
@@ -349,8 +347,7 @@ the specified package list.
 
 If there is no `requirements.txt` file or the `--force-generate` option is specified,
 the package dependencies will be determined from the current Python environment, or
-from an alternative Python executable specified via the `--python` option or via the
-`RETICULATE_PYTHON` environment variable:
+from an alternative Python executable specified via the `--python` option:
 
 ```bash
 rsconnect deploy api --python /path/to/python my-api/
@@ -371,8 +368,7 @@ manifest in a later deployment. Use the `write-manifest` command to do this.
 The `write-manifest` command will also create a `requirements.txt` file, if it does
 not already exist or the `--force-generate` option is specified. It will contain
 the package dependencies from the current Python environment, or from an alternative
-Python executable specified in the `--python` option or via the `RETICULATE_PYTHON`
-environment variable.
+Python executable specified in the `--python` option.
 
 Here is an example of the `write-manifest` command:
 
diff --git a/rsconnect/actions.py b/rsconnect/actions.py
index 46c994ba..46ca1161 100644
--- a/rsconnect/actions.py
+++ b/rsconnect/actions.py
@@ -113,7 +113,6 @@ def which_python(python, env=os.environ):
 
     In priority order:
     * --python specified on the command line
-    * RETICULATE_PYTHON defined in the environment
     * the python binary running this script
     """
     warn("This method has been moved and will be deprecated.", DeprecationWarning, stacklevel=2)
@@ -122,9 +121,6 @@ def which_python(python, env=os.environ):
             raise RSConnectException('The file, "%s", does not exist or is not executable.' % python)
         return python
 
-    if "RETICULATE_PYTHON" in env:
-        return os.path.expanduser(env["RETICULATE_PYTHON"])
-
     return sys.executable
 
 
diff --git a/rsconnect/bundle.py b/rsconnect/bundle.py
index 2149001a..b9783c40 100644
--- a/rsconnect/bundle.py
+++ b/rsconnect/bundle.py
@@ -1080,7 +1080,6 @@ def which_python(python, env=os.environ):
 
     In priority order:
     * --python specified on the command line
-    * RETICULATE_PYTHON defined in the environment
     * the python binary running this script
     """
     if python:
@@ -1088,9 +1087,6 @@ def which_python(python, env=os.environ):
             raise RSConnectException('The file, "%s", does not exist or is not executable.' % python)
         return python
 
-    if "RETICULATE_PYTHON" in env:
-        return os.path.expanduser(env["RETICULATE_PYTHON"])
-
     return sys.executable
 
 
diff --git a/tests/test_bundle.py b/tests/test_bundle.py
index 5c451bb4..a3276c4d 100644
--- a/tests/test_bundle.py
+++ b/tests/test_bundle.py
@@ -652,7 +652,6 @@ def test_which_python(self):
 
         self.assertEqual(which_python(sys.executable), sys.executable)
         self.assertEqual(which_python(None), sys.executable)
-        self.assertEqual(which_python(None, {"RETICULATE_PYTHON": "fake-python"}), "fake-python")
 
     def test_default_title(self):
         self.assertEqual(_default_title("testing.txt"), "testing")