diff --git a/packages/tracing-internal/src/node/integrations/express.ts b/packages/tracing-internal/src/node/integrations/express.ts
index f7ff20fa9986..eb1c22cf41d1 100644
--- a/packages/tracing-internal/src/node/integrations/express.ts
+++ b/packages/tracing-internal/src/node/integrations/express.ts
@@ -543,7 +543,9 @@ export function preventDuplicateSegments(
   reconstructedRoute?: string,
   layerPath?: string,
 ): string | undefined {
-  const originalUrlSplit = originalUrl?.split('/').filter(v => !!v);
+  // filter query params
+  const normalizeURL = stripUrlQueryAndFragment(originalUrl || '');
+  const originalUrlSplit = normalizeURL?.split('/').filter(v => !!v);
   let tempCounter = 0;
   const currentOffset = reconstructedRoute?.split('/').filter(v => !!v).length || 0;
   const result = layerPath
diff --git a/packages/tracing-internal/test/node/express.test.ts b/packages/tracing-internal/test/node/express.test.ts
index 4b8d31fb2cdc..1631971d9863 100644
--- a/packages/tracing-internal/test/node/express.test.ts
+++ b/packages/tracing-internal/test/node/express.test.ts
@@ -35,6 +35,22 @@ describe('unit Test for preventDuplicateSegments', () => {
     const result1 = preventDuplicateSegments(originalUrl, reconstructedRoute, layerPath);
     expect(result1).toBe('1234');
   });
+
+  it('should prevent duplicate segment v1 originalUrl with query param without trailing slash', () => {
+    const originalUrl = '/api/v1/1234?queryParam=123';
+    const reconstructedRoute = '/api/v1';
+    const layerPath = '/v1/1234';
+    const result1 = preventDuplicateSegments(originalUrl, reconstructedRoute, layerPath);
+    expect(result1).toBe('1234');
+  });
+
+  it('should prevent duplicate segment v1 originalUrl with query param with trailing slash', () => {
+    const originalUrl = '/api/v1/1234/?queryParam=123';
+    const reconstructedRoute = '/api/v1';
+    const layerPath = '/v1/1234';
+    const result1 = preventDuplicateSegments(originalUrl, reconstructedRoute, layerPath);
+    expect(result1).toBe('1234');
+  });
 });
 describe('preventDuplicateSegments should handle empty input gracefully', () => {
   it('Empty input values', () => {