diff --git a/dev-packages/e2e-tests/test-applications/node-nestjs/src/instrument.ts b/dev-packages/e2e-tests/test-applications/node-nestjs/src/instrument.ts
new file mode 100644
index 000000000000..09376810454f
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-nestjs/src/instrument.ts
@@ -0,0 +1,9 @@
+import * as Sentry from '@sentry/node';
+
+Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  tunnel: `http://localhost:3031/`, // proxy server
+  tracesSampleRate: 1,
+  tracePropagationTargets: ['http://localhost:3030', '/external-allowed'],
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-nestjs/src/main.ts b/dev-packages/e2e-tests/test-applications/node-nestjs/src/main.ts
index b168cc19e7c3..39bfec94b507 100644
--- a/dev-packages/e2e-tests/test-applications/node-nestjs/src/main.ts
+++ b/dev-packages/e2e-tests/test-applications/node-nestjs/src/main.ts
@@ -1,3 +1,7 @@
+// Import this first
+import './instrument';
+
+// Import other modules
 import { BaseExceptionFilter, HttpAdapterHost, NestFactory } from '@nestjs/core';
 import * as Sentry from '@sentry/node';
 import { AppModule1, AppModule2 } from './app.module';
@@ -6,14 +10,6 @@ const app1Port = 3030;
 const app2Port = 3040;
 
 async function bootstrap() {
-  Sentry.init({
-    environment: 'qa', // dynamic sampling bias to keep transactions
-    dsn: process.env.E2E_TEST_DSN,
-    tunnel: `http://localhost:3031/`, // proxy server
-    tracesSampleRate: 1,
-    tracePropagationTargets: ['http://localhost:3030', '/external-allowed'],
-  });
-
   const app1 = await NestFactory.create(AppModule1);
 
   const { httpAdapter } = app1.get(HttpAdapterHost);
diff --git a/dev-packages/e2e-tests/test-applications/node-nestjs/tests/transactions.test.ts b/dev-packages/e2e-tests/test-applications/node-nestjs/tests/transactions.test.ts
index 3fe5c1e65263..08a3998d0ecd 100644
--- a/dev-packages/e2e-tests/test-applications/node-nestjs/tests/transactions.test.ts
+++ b/dev-packages/e2e-tests/test-applications/node-nestjs/tests/transactions.test.ts
@@ -101,6 +101,24 @@ test('Sends an API route transaction', async ({ baseURL }) => {
           trace_id: expect.any(String),
           origin: 'manual',
         },
+        {
+          span_id: expect.any(String),
+          trace_id: expect.any(String),
+          data: {
+            'sentry.origin': 'manual',
+            component: '@nestjs/core',
+            'nestjs.version': expect.any(String),
+            'nestjs.type': 'handler',
+            'nestjs.callback': 'testTransaction',
+            'otel.kind': 'INTERNAL',
+          },
+          description: 'testTransaction',
+          parent_span_id: expect.any(String),
+          start_timestamp: expect.any(Number),
+          timestamp: expect.any(Number),
+          status: 'ok',
+          origin: 'manual',
+        },
       ]),
       transaction: 'GET /test-transaction',
       type: 'transaction',