diff --git a/.github/workflows/build.yml b/.github/workflows/build.yml
index d617b09bea01..bcbef8e06a8e 100644
--- a/.github/workflows/build.yml
+++ b/.github/workflows/build.yml
@@ -1004,6 +1004,7 @@ jobs:
             'create-remix-app-express-vite-dev',
             'debug-id-sourcemaps',
             'node-express-esm-loader',
+            'node-express-esm-without-loader',
             'nextjs-app-dir',
             'nextjs-14',
             'react-create-hash-router',
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/.npmrc b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/.npmrc
new file mode 100644
index 000000000000..070f80f05092
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/.npmrc
@@ -0,0 +1,2 @@
+@sentry:registry=http://127.0.0.1:4873
+@sentry-internal:registry=http://127.0.0.1:4873
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/package.json b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/package.json
new file mode 100644
index 000000000000..b339fa65d2a2
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/package.json
@@ -0,0 +1,24 @@
+{
+  "name": "node-express-esm-without-loader",
+  "version": "1.0.0",
+  "private": true,
+  "scripts": {
+    "start": "node src/app.mjs",
+    "clean": "npx rimraf node_modules pnpm-lock.yaml",
+    "test:build": "pnpm install",
+    "test:assert": "playwright test"
+  },
+  "dependencies": {
+    "@sentry/node": "latest || *",
+    "@sentry/opentelemetry": "latest || *",
+    "express": "4.19.2"
+  },
+  "devDependencies": {
+    "@sentry-internal/event-proxy-server": "link:../../../event-proxy-server",
+    "@playwright/test": "^1.27.1"
+  },
+  "volta": {
+    "extends": "../../package.json",
+    "node": "18.19.1"
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/playwright.config.ts b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/playwright.config.ts
new file mode 100644
index 000000000000..5e672ed97676
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/playwright.config.ts
@@ -0,0 +1,70 @@
+import type { PlaywrightTestConfig } from '@playwright/test';
+import { devices } from '@playwright/test';
+
+// Fix urls not resolving to localhost on Node v17+
+// See: https://github.com/axios/axios/issues/3821#issuecomment-1413727575
+import { setDefaultResultOrder } from 'dns';
+setDefaultResultOrder('ipv4first');
+
+const eventProxyPort = 3031;
+const expressPort = 3030;
+
+/**
+ * See https://playwright.dev/docs/test-configuration.
+ */
+const config: PlaywrightTestConfig = {
+  testDir: './tests',
+  /* Maximum time one test can run for. */
+  timeout: 150_000,
+  expect: {
+    /**
+     * Maximum time expect() should wait for the condition to be met.
+     * For example in `await expect(locator).toHaveText();`
+     */
+    timeout: 5000,
+  },
+  /* Run tests in files in parallel */
+  fullyParallel: true,
+  /* Fail the build on CI if you accidentally left test.only in the source code. */
+  forbidOnly: !!process.env.CI,
+  /* Retry on CI only */
+  retries: 0,
+  /* Reporter to use. See https://playwright.dev/docs/test-reporters */
+  reporter: 'list',
+  /* Shared settings for all the projects below. See https://playwright.dev/docs/api/class-testoptions. */
+  use: {
+    /* Maximum time each action such as `click()` can take. Defaults to 0 (no limit). */
+    actionTimeout: 0,
+
+    /* Base URL to use in actions like `await page.goto('/')`. */
+    baseURL: `http://localhost:${expressPort}`,
+  },
+
+  /* Configure projects for major browsers */
+  projects: [
+    {
+      name: 'chromium',
+      use: {
+        ...devices['Desktop Chrome'],
+      },
+    },
+  ],
+
+  /* Run your local dev server before starting the tests */
+  webServer: [
+    {
+      command: 'node start-event-proxy.mjs',
+      port: eventProxyPort,
+      stdout: 'pipe',
+      stderr: 'pipe',
+    },
+    {
+      command: 'pnpm start',
+      port: expressPort,
+      stdout: 'pipe',
+      stderr: 'pipe',
+    },
+  ],
+};
+
+export default config;
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/app.mjs b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/app.mjs
new file mode 100644
index 000000000000..0d318ab5fc13
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/app.mjs
@@ -0,0 +1,46 @@
+import './instrument.mjs';
+
+// Below other imports
+import * as Sentry from '@sentry/node';
+import express from 'express';
+
+const app = express();
+const port = 3030;
+
+app.get('/test-success', function (req, res) {
+  setTimeout(() => {
+    res.status(200).end();
+  }, 100);
+});
+
+app.get('/test-params/:param', function (req, res) {
+  const { param } = req.params;
+  Sentry.setTag(`param-${param}`, 'yes');
+  Sentry.captureException(new Error(`Error for param ${param}`));
+
+  setTimeout(() => {
+    res.status(200).end();
+  }, 100);
+});
+
+app.get('/test-error', function (req, res) {
+  Sentry.captureException(new Error('This is an error'));
+  setTimeout(() => {
+    Sentry.flush(2000).then(() => {
+      res.status(200).end();
+    });
+  }, 100);
+});
+
+Sentry.setupExpressErrorHandler(app);
+
+app.use(function onError(err, req, res, next) {
+  // The error id is attached to `res.sentry` to be returned
+  // and optionally displayed to the user for support.
+  res.statusCode = 500;
+  res.end(res.sentry + '\n');
+});
+
+app.listen(port, () => {
+  console.log(`Example app listening on port ${port}`);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/instrument.mjs b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/instrument.mjs
new file mode 100644
index 000000000000..1636d10e836c
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/src/instrument.mjs
@@ -0,0 +1,7 @@
+import * as Sentry from '@sentry/node';
+
+Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  tunnel: `http://localhost:3031/`, // proxy server
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/start-event-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/start-event-proxy.mjs
new file mode 100644
index 000000000000..df0fdb65c929
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/start-event-proxy.mjs
@@ -0,0 +1,6 @@
+import { startEventProxyServer } from '@sentry-internal/event-proxy-server';
+
+startEventProxyServer({
+  port: 3031,
+  proxyServerName: 'node-express-esm-without-loader',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/tests/server.test.ts b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/tests/server.test.ts
new file mode 100644
index 000000000000..c2c076f13e4e
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-express-esm-without-loader/tests/server.test.ts
@@ -0,0 +1,35 @@
+import { expect, test } from '@playwright/test';
+import { waitForError } from '@sentry-internal/event-proxy-server';
+
+test('Should record exceptions captured inside handlers', async ({ request }) => {
+  const errorEventPromise = waitForError('node-express-esm-without-loader', errorEvent => {
+    return !!errorEvent?.exception?.values?.[0]?.value?.includes('This is an error');
+  });
+
+  await request.get('/test-error');
+
+  await expect(errorEventPromise).resolves.toBeDefined();
+});
+
+test('Isolates requests', async ({ request }) => {
+  const errorEventPromise = waitForError('node-express-esm-without-loader', errorEvent => {
+    return !!errorEvent?.exception?.values?.[0]?.value?.includes('Error for param 1');
+  });
+
+  const errorEventPromise2 = waitForError('node-express-esm-without-loader', errorEvent => {
+    return !!errorEvent?.exception?.values?.[0]?.value?.includes('Error for param 2');
+  });
+
+  await request.get('/test-params/1');
+  await request.get('/test-params/2');
+
+  const errorEvent1 = await errorEventPromise;
+  const errorEvent2 = await errorEventPromise2;
+
+  expect(errorEvent1.tags).toEqual({ 'param-1': 'yes' });
+  expect(errorEvent2.tags).toEqual({ 'param-2': 'yes' });
+
+  // Transaction is not set, since we have no expressIntegration by default
+  expect(errorEvent1.transaction).toBeUndefined();
+  expect(errorEvent2.transaction).toBeUndefined();
+});