diff --git a/dev-packages/node-integration-tests/suites/tracing-experimental/mongodb/scenario.js b/dev-packages/node-integration-tests/suites/tracing-experimental/mongodb/scenario.js
index 74213908f8e0..360c943e0724 100644
--- a/dev-packages/node-integration-tests/suites/tracing-experimental/mongodb/scenario.js
+++ b/dev-packages/node-integration-tests/suites/tracing-experimental/mongodb/scenario.js
@@ -5,10 +5,12 @@ Sentry.init({
   dsn: 'https://public@dsn.ingest.sentry.io/1337',
   release: '1.0',
   tracesSampleRate: 1.0,
-  debug: true,
   transport: loggingTransport,
 });
 
+// Stop the process from exiting before the transaction is sent
+setInterval(() => {}, 1000);
+
 // Must be required after Sentry is initialized
 const { MongoClient } = require('mongodb');
 
@@ -40,8 +42,6 @@ async function run() {
       }
     },
   );
-
-  Sentry.flush(2000);
 }
 
 // eslint-disable-next-line @typescript-eslint/no-floating-promises
diff --git a/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withConnect.js b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withConnect.js
new file mode 100644
index 000000000000..0d1b517209a0
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withConnect.js
@@ -0,0 +1,40 @@
+const { loggingTransport } = require('@sentry-internal/node-integration-tests');
+const Sentry = require('@sentry/node-experimental');
+
+Sentry.init({
+  dsn: 'https://public@dsn.ingest.sentry.io/1337',
+  release: '1.0',
+  tracesSampleRate: 1.0,
+  transport: loggingTransport,
+});
+
+// Stop the process from exiting before the transaction is sent
+setInterval(() => {}, 1000);
+
+const mysql = require('mysql');
+
+const connection = mysql.createConnection({
+  user: 'root',
+  password: 'docker',
+});
+
+connection.connect(function (err) {
+  if (err) {
+    return;
+  }
+});
+
+Sentry.startSpanManual(
+  {
+    op: 'transaction',
+    name: 'Test Transaction',
+  },
+  span => {
+    connection.query('SELECT 1 + 1 AS solution', function () {
+      connection.query('SELECT NOW()', ['1', '2'], () => {
+        span.end();
+        connection.end();
+      });
+    });
+  },
+);
diff --git a/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutCallback.js b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutCallback.js
new file mode 100644
index 000000000000..cd35953b0504
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutCallback.js
@@ -0,0 +1,45 @@
+const { loggingTransport } = require('@sentry-internal/node-integration-tests');
+const Sentry = require('@sentry/node-experimental');
+
+Sentry.init({
+  dsn: 'https://public@dsn.ingest.sentry.io/1337',
+  release: '1.0',
+  tracesSampleRate: 1.0,
+  transport: loggingTransport,
+});
+
+// Stop the process from exiting before the transaction is sent
+setInterval(() => {}, 1000);
+
+const mysql = require('mysql');
+
+const connection = mysql.createConnection({
+  user: 'root',
+  password: 'docker',
+});
+
+connection.connect(function (err) {
+  if (err) {
+    return;
+  }
+});
+
+Sentry.startSpan(
+  {
+    op: 'transaction',
+    name: 'Test Transaction',
+  },
+  span => {
+    const query = connection.query('SELECT 1 + 1 AS solution');
+    const query2 = connection.query('SELECT NOW()', ['1', '2']);
+
+    query.on('end', () => {
+      query2.on('end', () => {
+        // Wait a bit to ensure the queries completed
+        setTimeout(() => {
+          span.end();
+        }, 500);
+      });
+    });
+  },
+);
diff --git a/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutConnect.js b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutConnect.js
new file mode 100644
index 000000000000..844b25d63f2d
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/scenario-withoutConnect.js
@@ -0,0 +1,34 @@
+const { loggingTransport } = require('@sentry-internal/node-integration-tests');
+const Sentry = require('@sentry/node-experimental');
+
+Sentry.init({
+  dsn: 'https://public@dsn.ingest.sentry.io/1337',
+  release: '1.0',
+  tracesSampleRate: 1.0,
+  transport: loggingTransport,
+});
+
+// Stop the process from exiting before the transaction is sent
+setInterval(() => {}, 1000);
+
+const mysql = require('mysql');
+
+const connection = mysql.createConnection({
+  user: 'root',
+  password: 'docker',
+});
+
+Sentry.startSpanManual(
+  {
+    op: 'transaction',
+    name: 'Test Transaction',
+  },
+  span => {
+    connection.query('SELECT 1 + 1 AS solution', function () {
+      connection.query('SELECT NOW()', ['1', '2'], () => {
+        span.end();
+        connection.end();
+      });
+    });
+  },
+);
diff --git a/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/test.ts b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/test.ts
new file mode 100644
index 000000000000..43c67c9c8b07
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing-experimental/mysql/test.ts
@@ -0,0 +1,94 @@
+import { conditionalTest } from '../../../utils';
+import { createRunner } from '../../../utils/runner';
+
+conditionalTest({ min: 14 })('mysql auto instrumentation', () => {
+  test('should auto-instrument `mysql` package when using connection.connect()', done => {
+    const EXPECTED_TRANSACTION = {
+      transaction: 'Test Transaction',
+      spans: expect.arrayContaining([
+        expect.objectContaining({
+          description: 'SELECT 1 + 1 AS solution',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+        expect.objectContaining({
+          description: 'SELECT NOW()',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+      ]),
+    };
+
+    createRunner(__dirname, 'scenario-withConnect.js').expect({ transaction: EXPECTED_TRANSACTION }).start(done);
+  });
+
+  test('should auto-instrument `mysql` package when using query without callback', done => {
+    const EXPECTED_TRANSACTION = {
+      transaction: 'Test Transaction',
+      spans: expect.arrayContaining([
+        expect.objectContaining({
+          description: 'SELECT 1 + 1 AS solution',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+        expect.objectContaining({
+          description: 'SELECT NOW()',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+      ]),
+    };
+
+    createRunner(__dirname, 'scenario-withoutCallback.js').expect({ transaction: EXPECTED_TRANSACTION }).start(done);
+  });
+
+  test('should auto-instrument `mysql` package without connection.connect()', done => {
+    const EXPECTED_TRANSACTION = {
+      transaction: 'Test Transaction',
+      spans: expect.arrayContaining([
+        expect.objectContaining({
+          description: 'SELECT 1 + 1 AS solution',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+        expect.objectContaining({
+          description: 'SELECT NOW()',
+          op: 'db',
+          data: expect.objectContaining({
+            'db.system': 'mysql',
+            'net.peer.name': 'localhost',
+            'net.peer.port': 3306,
+            'db.user': 'root',
+          }),
+        }),
+      ]),
+    };
+
+    createRunner(__dirname, 'scenario-withoutConnect.js').expect({ transaction: EXPECTED_TRANSACTION }).start(done);
+  });
+});