diff --git a/src/lib/libwebaudio.js b/src/lib/libwebaudio.js
index 9d13142700c47..6103c017c6e8f 100644
--- a/src/lib/libwebaudio.js
+++ b/src/lib/libwebaudio.js
@@ -183,7 +183,13 @@ let LibraryWebAudio = {
 
     // TODO: In MINIMAL_RUNTIME builds, read this file off of a preloaded Blob,
     // and/or embed from a string like with WASM_WORKERS==2 mode.
-    audioWorklet.addModule('{{{ TARGET_BASENAME }}}.aw.js').then(() => {
+    audioWorklet.addModule(
+#if MINIMAL_RUNTIME
+      '{{{ TARGET_BASENAME }}}.aw.js'
+#else
+      locateFile('{{{ TARGET_BASENAME }}}.aw.js')
+#endif
+    ).then(() => {
 #if WEBAUDIO_DEBUG
       console.log(`emscripten_start_wasm_audio_worklet_thread_async() addModule('audioworklet.js') completed`);
 #endif
diff --git a/test/test_browser.py b/test/test_browser.py
index 15d40867f7c68..87e4facdee1a8 100644
--- a/test/test_browser.py
+++ b/test/test_browser.py
@@ -5521,6 +5521,22 @@ def test_full_js_library_strict(self):
   def test_audio_worklet(self, args):
     self.btest_exit('webaudio/audioworklet.c', emcc_args=['-sAUDIO_WORKLET', '-sWASM_WORKERS', '-DTEST_AND_EXIT'] + args)
 
+  # Test that when using audio worklets, it is possible to use the locateFile() machinery to reroute where the .aw.js file is loaded from.
+  @requires_sound_hardware
+  def test_audio_worklet_locatefile(self):
+    # Create a locateFile() function that loads .aw.js from a subdirectory.
+    create_file('shell.html', read_file(path_from_root('src/shell.html')).replace('var Module = {', '''
+    var Module = {
+      locateFile: function(path, prefix) {
+        return path.includes('.aw.js') ? 'subdir/' + path : path;
+      },
+    '''))
+
+    self.compile_btest('webaudio/audioworklet.c', ['--shell-file', 'shell.html', '-sAUDIO_WORKLET', '-sWASM_WORKERS', '-DTEST_AND_EXIT', '-o', 'test.html'])
+    ensure_dir('subdir')
+    shutil.move('test.aw.js', Path('subdir/test.aw.js'))
+    self.run_browser('test.html', '/report_result?exit:0')
+
   # Tests that audioworklets and workers can be used at the same time
   # Note: doesn't need audio hardware (and has no AW code that tests 2GB or wasm64)
   def test_audio_worklet_worker(self):