diff --git a/src/Microsoft.Android.Runtime.NativeAOT/Android.Runtime.NativeAOT/JavaInteropRuntime.cs b/src/Microsoft.Android.Runtime.NativeAOT/Android.Runtime.NativeAOT/JavaInteropRuntime.cs
index 982f1c5a210..340078864e8 100644
--- a/src/Microsoft.Android.Runtime.NativeAOT/Android.Runtime.NativeAOT/JavaInteropRuntime.cs
+++ b/src/Microsoft.Android.Runtime.NativeAOT/Android.Runtime.NativeAOT/JavaInteropRuntime.cs
@@ -31,7 +31,7 @@ static void JNI_OnUnload (IntPtr vm, IntPtr reserved)
 
 	// symbol name from `$(IntermediateOutputPath)obj/Release/osx-arm64/h-classes/net_dot_jni_hello_JavaInteropRuntime.h`
 	[UnmanagedCallersOnly (EntryPoint="Java_net_dot_jni_nativeaot_JavaInteropRuntime_init")]
-	static void init (IntPtr jnienv, IntPtr klass)
+	static void init (IntPtr jnienv, IntPtr klass, IntPtr classLoader)
 	{
 		JniTransition   transition  = default;
 		try {
@@ -41,6 +41,7 @@ static void init (IntPtr jnienv, IntPtr klass)
 			var typeManager = new ManagedTypeManager ();
 			var options = new NativeAotRuntimeOptions {
 				EnvironmentPointer          = jnienv,
+				ClassLoader                 = new JniObjectReference (classLoader),
 				TypeManager                 = typeManager,
 				ValueManager                = new ManagedValueManager (),
 				UseMarshalMemberBuilder     = false,
diff --git a/src/Xamarin.Android.Build.Tasks/Resources/JavaInteropRuntime.java b/src/Xamarin.Android.Build.Tasks/Resources/JavaInteropRuntime.java
index cb0474682f9..194c8ddb03d 100644
--- a/src/Xamarin.Android.Build.Tasks/Resources/JavaInteropRuntime.java
+++ b/src/Xamarin.Android.Build.Tasks/Resources/JavaInteropRuntime.java
@@ -11,5 +11,5 @@ public class JavaInteropRuntime {
     private JavaInteropRuntime() {
     }
 
-    public static native void init();
+    public static native void init(ClassLoader classLoader);
 }
diff --git a/src/Xamarin.Android.Build.Tasks/Resources/NativeAotRuntimeProvider.java b/src/Xamarin.Android.Build.Tasks/Resources/NativeAotRuntimeProvider.java
index a3acd8935af..f5db2335339 100644
--- a/src/Xamarin.Android.Build.Tasks/Resources/NativeAotRuntimeProvider.java
+++ b/src/Xamarin.Android.Build.Tasks/Resources/NativeAotRuntimeProvider.java
@@ -37,8 +37,10 @@ public void attachInfo(android.content.Context context, android.content.pm.Provi
             Log.e(TAG, "Failed to set environment variables", e);
         }
 
+        ClassLoader loader  = context.getClassLoader ();
+
         // Initialize .NET runtime
-        JavaInteropRuntime.init();
+        JavaInteropRuntime.init(loader);
         // NOTE: only required for custom applications
         ApplicationRegistration.registerApplications();
         super.attachInfo (context, info);
diff --git a/tests/Mono.Android-Tests/Mono.Android-Tests/Java.Interop/JnienvTest.cs b/tests/Mono.Android-Tests/Mono.Android-Tests/Java.Interop/JnienvTest.cs
index c97a8d6e1d3..a563f56fd3a 100644
--- a/tests/Mono.Android-Tests/Mono.Android-Tests/Java.Interop/JnienvTest.cs
+++ b/tests/Mono.Android-Tests/Mono.Android-Tests/Java.Interop/JnienvTest.cs
@@ -44,7 +44,7 @@ public void TestMyPaintColor ()
 		public void RegisterTypeOnNewNativeThread ()
 		{
 			Java.Lang.JavaSystem.LoadLibrary ("reuse-threads");
-			int ret = rt_register_type_on_new_thread ("from.NewThreadOne", Application.Context.ClassLoader.Handle);
+			int ret = rt_register_type_on_new_thread ("from.NewNativeThreadOne", Application.Context.ClassLoader.Handle);
 			Assert.AreEqual (0, ret, $"Java type registration on a new thread failed with code {ret}");
 		}
 
@@ -57,6 +57,23 @@ public void RegisterTypeOnNewJavaThread ()
 			Assert.AreNotEqual (null, thread.Instance, "Failed to register instance of a class on new thread");
 		}
 
+		[Test]
+		public void RegisterTypeOnNewManagedThread ()
+		{
+			Exception? ex = null;
+			var thread = new System.Threading.Thread (() => {
+				try {
+					using var instance = new RegisterMeOnNewManagedThreadOne ();
+				}
+				catch (Exception e) {
+					ex = e;
+				}
+			});
+			thread.Start ();
+			thread.Join (5000);
+			Assert.IsNull (ex, $"Failed to register instance of a class on new thread: {ex}");
+		}
+
 		[Test]
 		public void ThreadReuse ()
 		{
@@ -459,8 +476,12 @@ public void DoNotLeakWeakReferences ()
 		}
 	}
 
-	[Register ("from/NewThreadOne")]
-	class RegisterMeOnNewThreadOne : Java.Lang.Object
+	[Register ("from/NewNativeThreadOne")]
+	class RegisterMeOnNewNativeThreadOne : Java.Lang.Object
+	{}
+
+	[Register ("from/NewManagedThreadOne")]
+	class RegisterMeOnNewManagedThreadOne : Java.Lang.Object
 	{}
 
 	[Register ("from/NewThreadTwo")]